FastQCFastQC Report
Mon 6 Feb 2012
N158.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename N158.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11380422
Filtered Sequences 0
Sequence length 50
%GC 48

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC 2189744 19.24132514593923 Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG 648118 5.695026071968157 TruSeq Adapter, Index 1 (100% over 49bp)
TAGCTTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCC 590627 5.1898514835390115 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACAAGATCGGAAGAGCACACGTCTGAACT 161030 1.4149738911263572 Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCACAGTGGCTAAGTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAG 133796 1.1756681782099119 Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT 111747 0.9819231659423525 Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT 72311 0.6353982303995406 Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTCCAGT 69497 0.6106715550618422 Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGCTTATCAGACTGATGTTGACAGATCGGAAGAGAACACGTCTGAACTC 66828 0.5872189976786449 Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TAGCTTATCAGACTGATGTTGAGATCGGAAGAGCACACGTCTGAACTCCA 61558 0.5409114002978097 Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA 55695 0.48939309983408347 Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGAACTGAATTCCATAGGCTGTAGATCGGAAGAGCACACGTCTGAACT 45966 0.4039041785972436 Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC 45028 0.3956619534846774 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTAGATCGGAAGAGCACACGTCTGAACTCC 44166 0.38808754192067746 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG 43779 0.3846869650352157 Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGAGTTCTACAGTCCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC 40796 0.3584752832539953 Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAGCACCATTTGAAATCAGTGTTAGATCGGAAGAGCACACGTCTGAACTC 40158 0.35286916425419024 Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT 39446 0.3466128057465707 TruSeq Adapter, Index 1 (100% over 48bp)
AAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA 37338 0.3280897667942366 Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC 31550 0.27723049285870066 TruSeq Adapter, Index 1 (100% over 50bp)
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCC 30750 0.2702008765580046 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTAGATCGGAAGAGCACACGTCTGAACT 30271 0.26599189379796284 Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC 29350 0.25789904803178654 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGAGTTCTACAGTCCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA 28933 0.2542348605350487 Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGAGGTAGTAGGTTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA 28588 0.2512033385053735 Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGAAAGATCGGAAGAGCACACGTCTGAACTC 28325 0.2488923521465197 Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC 27059 0.23776798435066818 Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGA 24388 0.21429785292671924 TruSeq Adapter, Index 1 (100% over 40bp)
CAGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTC 23947 0.2104227769409605 Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCTACAGTCCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCA 23755 0.20873566902879348 TruSeq Adapter, Index 1 (100% over 37bp)
CTTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTCCAG 23017 0.20225084799140136 Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTC 22526 0.1979364209868492 TruSeq Adapter, Index 1 (100% over 50bp)
TTCACAGTGGCTAAGTTCCGCAGATCGGAAGAGCACACGTCTGAACTCCA 22463 0.19738283870316936 Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTATCAGACTGATGTTGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC 21990 0.19322657806538282 Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAGCCTATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTC 20988 0.184421983648761 Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA 20875 0.18342905034628768 Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGCTTATCAGACTGATGTTAGATCGGAAGAGCACACGTCTGAACTCCAG 20875 0.18342905034628768 Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGTTCTACAGTCCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA 19333 0.16987946492669606 Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC 18722 0.16451059547703942 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTCTACAGTCCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT 18568 0.16315739433915544 TruSeq Adapter, Index 1 (100% over 35bp)
TAGCNTATCAGACTGATGTTGACAGATCGGAAGAGAACACGTCTGAACTC 17582 0.15449339224854755 Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TGGCTCAGTTCAGCAGGAACAGATCGGAAGAGCACACGTCTGAACTCCAG 17010 0.14946721659354986 Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC 16730 0.14700685088830626 Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA 16520 0.14516157660937354 Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGAACTGAATTCCATAGGTTAGATCGGAAGAGCACACGTCTGAACTCC 15985 0.14046052070828305 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGTTCTACAGTCCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC 15054 0.13227980473834802 Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA 15011 0.1319019628621856 Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCACAGTGGCTAAGTTCCGTAGATCGGAAGAGCACACGTCTGAACTCCA 14854 0.130522400663174 Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCTTATCAGACTGATGTTGGAGATCGGAAGAGCACACGTCTGAACTCC 14473 0.1271745458999675 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGAGTTCTACAGTCCGAAGATCGGAAGAGCACACGTCTGAACTCCAGT 13911 0.12223624044872854 Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGCCTATCAGACTGATGTTGACAGATCGGAAGAGAACACGTCTGAACTC 13575 0.11928380160243618 Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TTCACAGTGGCTAAGTTCCGAAGATCGGAAGAGCACACGTCTGAACTCCA 13499 0.11861598805387005 Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC 13207 0.11605017810411598 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACGAGATCGGAAGAGCACACGTCTGAACT 12931 0.11362496048037585 Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC 12633 0.1110064284083666 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGCGTATCAGACTGATGTTGACAGATCGGAAGAGAACACGTCTGAACTC 12542 0.1102068095541624 Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
TATCAGACTGATGTTGACAGATCGGAAGAGCACACGTCTGAACTCCAGTC 11899 0.10455675545247795 Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGCTCAGTTCAGCAGGAACAGAGATCGGAAGAGCACACGTCTGAACTCC 11523 0.10125283579115081 Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GTCTG 10654955 32.880314 430.89066 41
CGTCT 10602310 32.769024 431.46652 40
TCTGA 10761735 26.466146 342.5599 42
GATCG 10962165 22.898014 295.32434 25
ATCGG 10914620 22.798702 295.74243 26
TCGGA 10879630 22.725613 287.09897 27
CACGT 10631975 22.243036 291.08246 38
ACGTC 10617420 22.212585 291.26028 39
AGATC 10878535 18.10906 234.42958 24
CTGAA 10765545 17.92097 232.12917 43
GAACT 10638645 17.709726 231.2151 45
TGAAC 10543275 17.550966 231.71779 44
AACTC 9974210 16.629633 231.29012 46
GGAAG 11020240 15.557182 194.46826 29
CGGAA 10824765 15.305132 194.32715 28
TGTTG 4215715 15.292689 652.0785 17
ACACG 10689265 15.137185 197.57072 37
AGAGC 10615050 15.0086155 184.2085 32
AGCAC 10576060 14.976872 183.55165 34
GAGCA 10590435 14.973813 183.75723 33
GCACA 10480840 14.842031 183.06723 35
CACAC 10457780 14.832536 182.81035 36
ACTCC 6923970 14.508215 129.46266 46
GAAGA 12357435 13.902481 154.87424 30
AAGAG 10883495 12.244256 154.46825 31
ATGTT 4223330 12.209327 521.84155 16
CTCCA 5567050 11.664977 85.98169 25
TTATC 3921945 11.355777 500.3453 5
CTTAT 3791620 10.978428 499.071 4
CTGAT 4324110 10.634207 445.61267 13
CAGTC 5066780 10.600154 78.61617 28
GATGT 4294885 10.545843 445.396 15
TCCAG 5031135 10.525581 85.71764 26
TGATG 4282530 10.515507 444.78848 14
GTTGA 4279315 10.507612 437.96497 18
CCAGT 4455440 9.3211775 78.900246 27
GCTTA 3784370 9.306834 422.87888 3
GACTG 4433280 9.260334 379.16605 11
TAGCT 3753345 9.2305355 420.9574 1
AGCTT 3740205 9.19822 422.11566 2
TCAGA 5281365 8.7916765 305.70517 8
ATCAG 5036150 8.383476 302.65 7
TATCA 4173175 8.178979 355.32727 6
TTGAC 3297990 8.110689 341.5538 19
ACTGA 4511000 7.509281 301.27158 12
CAGAT 4441645 7.393829 212.47087 23
AGACT 4303040 7.1630983 302.31668 10
GTCAC 3153770 6.597967 78.55666 30
CAGAC 4296880 6.084858 256.6025 9
AGTCA 3530925 5.8777895 62.459198 29
TCACA 3218840 5.3666534 61.79246 31
TGACA 3203245 5.3323135 223.58208 20
CGATC 2228185 4.661561 81.19149 39
CACAT 2672625 4.4559693 60.672836 32
GACAG 3041240 4.3000083 175.78831 21
ACATC 2389730 3.984309 59.12334 33
TAGAT 2012575 3.9382741 29.961668 22
TCTCG 1245930 3.8508508 124.37973 42
ACGAT 2303920 3.8352435 63.735764 38
CTCGT 1172260 3.6231554 124.33712 43
ACAGA 3174080 3.5765188 140.0402 22
GTCCG 1299145 3.4104657 36.39452 9
CATCA 2041550 3.4038012 58.292515 34
TCACG 1619180 3.3874683 72.61411 36
GATCT 1372360 3.3750207 97.78703 40
ATCTC 1282930 3.1600213 99.11694 41
TCCGA 1507795 3.154441 28.416681 10
AAGAT 2374760 3.145507 47.98916 22
ATCAC 1867740 3.1140141 57.829807 35
CGTAT 1177625 2.8961124 99.122986 45
GTTCT 764255 2.776704 24.729992 1
TCGTA 1124850 2.7663238 99.25276 44
TTCTA 916660 2.6541388 37.58159 2
AGTCC 1256615 2.62895 28.494558 8
ACAGT 1569660 2.61295 28.384188 4
GTATG 1056920 2.5952065 99.28526 46
TCTAC 1052485 2.5924056 33.513252 3
GAGAT 1548035 2.572928 19.113438 20
AGTTC 935160 2.2998226 32.108627 14
CACGA 1581040 2.2389278 53.2903 37
TTGAA 1108580 2.1693063 69.504456 19
TACAG 1249270 2.0796094 22.473766 5
CTACA 1196370 1.9946637 22.352112 4
GTAGA 1128805 1.8761425 16.63997 20
TGAAG 1010770 1.679961 56.320076 20
CCGAC 934690 1.663487 21.593098 11
CGACG 915580 1.6269324 21.496971 12
GACGA 1081330 1.5288922 17.214998 13
GATCA 840830 1.399696 19.047018 16
TGGCT 435150 1.3428371 41.239452 8
TGTAG 516715 1.268764 10.304229 20
TTTGT 294005 1.2556666 22.177315 12
TTGTA 425095 1.2289174 29.231974 13
GTAGT 500030 1.227795 35.399445 5
AGTAG 680020 1.1302345 25.77725 7
TTAGA 553290 1.0826963 16.481634 21
TAGTA 547215 1.0708085 30.003305 6
GAGTT 431470 1.0594498 14.973218 3
GGTAG 505230 1.0536879 29.971987 4
TGAGA 623445 1.0362035 8.07005 1
GTTTG 281570 1.0214074 19.039042 11
TTCCG 323540 0.9999794 37.225983 16
GTTCC 301005 0.9303293 38.866222 15
GTGGC 348270 0.91283756 35.91102 7
CAGTG 418255 0.8736604 25.758215 5
TGAGG 408610 0.852181 28.37485 1
GTTAG 342530 0.8410627 18.34533 20
GGCTA 399680 0.8348605 27.891602 9
CGAGA 584340 0.82619816 12.449212 19
GCTGT 266780 0.82326114 12.938665 17
GAGGT 372930 0.77776814 28.555735 2
AGAGA 678435 0.7632595 10.414455 32
AGTGG 348965 0.72778773 27.860174 6
AAGTT 369775 0.72358805 25.483889 13
TGCTG 232960 0.7188954 13.163436 16
AGAAC 618070 0.69643456 10.206279 34
TTCAC 276290 0.68053776 28.619396 1
TGTAT 234515 0.67796504 26.91943 14
TAGTT 229135 0.66241187 14.444664 9
AGAGT 395875 0.6579682 10.266407 2
GTGCT 212905 0.6570073 13.419377 15
CTGTA 256585 0.63101494 7.7576294 21
TAAGT 319780 0.6257562 25.585636 12
GATTG 247080 0.60669076 15.599476 11
CTAAG 358075 0.59607303 22.10271 11
CCGAG 333220 0.59211254 14.732162 18
AGGTA 356210 0.5920426 22.586164 3
TCAGT 239750 0.58961296 7.82003 5
TTGTG 160970 0.58392566 18.024773 13
TGTTA 201205 0.5816684 9.321109 19
ATTGT 194715 0.56290627 17.968985 12
GCTAA 335815 0.5590178 22.075933 10
CAGAG 393220 0.55597365 7.714884 1
TTGAG 223920 0.5498227 9.952236 19
CACAG 382300 0.54137915 16.813784 3
ACAAG 474590 0.53476286 11.773108 22
TATAG 270700 0.5297148 17.721985 16
GAACA 469925 0.5295064 10.230095 35
TTCAG 207755 0.5109283 7.5793257 9
GAGAA 452725 0.509329 10.253411 33
ATTCT 174220 0.5044445 17.899473 1
TTTGA 173265 0.50089604 7.8735347 9
GGCTG 190415 0.4990897 9.468617 19
ATAGT 254930 0.49885556 17.111448 17
ATAGG 300055 0.49870962 12.551037 16
GTTGT 137390 0.49838826 14.179727 12
CTCAG 238110 0.49814728 6.3579435 4
CTGTT 136025 0.4942083 12.887156 18
CAAGA 434565 0.48966312 11.450855 23
GTATA 244545 0.4785338 17.894629 15
AGTTT 162775 0.47057018 14.459627 10
AACAC 408785 0.46133482 10.209017 36
GGTTG 149040 0.45920706 13.642685 11
AGGCT 219195 0.4578594 9.824776 18
AGATT 232825 0.45559973 11.992588 10
CAGTT 184705 0.45424175 7.457582 6
TTGGA 183880 0.4515068 5.67318 4
TGTGC 145330 0.44847646 13.576053 14
TTCCA 177560 0.4373531 12.953141 12
TAGGT 177730 0.4364058 10.688693 9
ATTCC 176360 0.4343973 13.039122 11
AGGTT 175450 0.4308074 10.950767 10
GTGTT 113925 0.41326794 9.035257 19
AACTG 241595 0.40217346 8.130676 5
TGAAT 201775 0.39484003 10.452795 8
TCCAT 159145 0.39199457 12.94854 13
AATTC 197770 0.3876081 10.382335 10
GTAGG 185430 0.38672554 9.4643955 8
TAGGC 183160 0.38258868 10.538336 17
GCTCA 182765 0.3823606 6.577416 3
GAATT 187780 0.36745417 10.420744 9
GGCTC 138235 0.36288923 7.5804744 2
GACAA 306095 0.3449045 11.745151 21
CATAG 204605 0.34059772 8.825596 15
ACTGT 137015 0.3369586 6.4508495 18
TCGGG 127060 0.33303222 6.0741587 27
TCAGC 155785 0.32591605 7.170376 10
GCCAG 182440 0.32418525 5.570923 6
TATGC 128995 0.31723514 6.8208327 46
GTTCA 127675 0.31398886 7.5263114 8
AGTGT 122185 0.30001825 6.3950863 18
ATTTG 99350 0.2872133 7.7255263 8
AGTTG 116645 0.2864151 7.4174385 9
CTATC 111510 0.27466345 9.3501 5
TGCCA 130255 0.27250504 6.096722 5
AGCCT 123580 0.25854033 7.8690047 2
TAGCA 151080 0.25149682 8.642443 1
CCATA 150600 0.25108987 8.74058 14
AGCTG 120125 0.2509198 6.522046 2
CTGCC 94885 0.249478 7.6870456 4
CCTAT 100170 0.24673156 9.188655 4
ATTGG 100000 0.24554425 5.30596 3
CATTT 84785 0.24549031 7.89322 7
TAGCC 116785 0.2443246 8.323968 1
GCTGC 84790 0.22258747 7.766371 3
GCAGG 125120 0.2219838 5.4681234 13
AGCGT 105100 0.21953522 5.769353 2
GCCTA 104645 0.21892664 7.919518 3
AGTTA 110475 0.21618116 5.9708443 19
GCGTA 102195 0.2134672 5.814454 3
AAGCT 122885 0.20456174 5.3723392 1
CCATT 77435 0.19073236 6.547969 6
TAGCG 82060 0.17140876 5.969463 1
GTATC 69105 0.16994873 6.795632 5